![]() New users and collaborators are always welcome please contact us for further information. Product testing, protocol development and collaborations are also available by agreement. Our expert staff are available to assist with flow training, flow data analysis, experimental planning, flow data acquisition and cell sorting or simply to provide advice. Laser cutter/etching (Universal Laser Systems: VLS3.50 Desktop).If you would like to utilise these systems please contact the flow team: 3D printer (3D Gence Medical: One) The Flow Facility also houses a 3D printer and a laser cutter for simple development and prototyping purposes. This site licence is available to users within the precinct and can be obtained by completing this online form. The Flow Facility also provides at cost, site licences for accessing the latest version of FlowJo. For information regarding the FACSymphony, please contact the flow team: BD FACSymphony A5 (5 laser/7 laser config: 28-30 parameter) The BD FACSymphony A5 has the capacity to analyse high dimensional panels (currently up to 28 fluorescent parameters). Beckman Coulter Cytoflex (tube analyser) (3 laser: 13 parameter).Beckman Coulter Cytoflex (plate analyser) (3 laser: 13 parameter).BD LSR Fortessa (5 laser: 17 parameter).BD FACS Canto II (3 laser: 9 parameter).Please contact the flow team ( ) to request access. These instruments are user operated to gain access to these instruments, you must be trained by a flow operator. These instruments are also bookable via the online Garvan Booking System. We have a range of flow cytometry analyser instruments from Becton Dickinson (BD) and Beckman Coulter as shown below. If you would like access to our sorter instruments or have any questions about our service capabilities please contact us via email. Our services include set up and operation of the instruments by our experienced sort operators. The LCA provides access and training on two FACS Calibur benchtop cytometers, one FACS Canto II. All our sorters are optimised to use 70um, 85um, 100um and 130um nozzles, depending on the size of your cells.Ĭell sorting instruments are bookable via the Garvan Booking System. Flow Cytometry & Cell Sorting Instruments and Services. We have the capability to sort in to numerous collection vessels including:- 15ml Tubes, 5ml FACS tubes, 1.8ml Eppendorf tubes, 12-wells up to 384-wells PCR/culture plates. These instruments are housed within certified hoods enabling researchers to sort samples derived from human origin in addition to samples of animal origin pending biosafety approval by Garvan Work, Health and Safety. The Flow Facility currently houses three BD cell sorters (Aria IIu and Aria III x 2) that have the capacity to sort cell populations in bulk, down to single cells in 384-well plates. We provides excellent cytometry services as well as expert knowledge in the field.Īs an integral part of The Garvan-Weizmann Centre for Cellular Genomics, the Flow Facility strives to expand its technological capabilities by supporting single cell technologies and expanding in high dimensional cytometry using cutting edge instrumentation such as the BD FACSymphony.
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